Fig. 3

Molecular interaction of mGluR1a with TRPC1 and 4 in HEK-293 cells, and with TRPC1 in hippocampus. a. Top left: Cell extracts from HEK-293 cells transfected with mGluR1a alone, or with mGluR1a and FLAG-TRPC1, were immunoprecipitated with anti-FLAG and immunodetected by Western blot using anti-mGluR1a (n = 3 separate experiments). Top right: Immunodetection of mGluR1a from cell extracts. Bottom left: Cell extracts from HEK-293 cells transfected with myc-TRPC4 alone, or with myc-TRPC4 and mGluR1a, were immunoprecipitated with anti-mGluR1a and immunodetected using anti-myc (n = 3). Bottom right: Immunodetection of myc-TRPC4 from cell extracts. b. Left: Whole hippocampus tissue extract was immunoprecipitated with anti-TRPC1 in absence or presence of TRPC1 blocking peptide and immunodetected using anti-mGluR1a (n = 3). Right: Immunodetection of mGluR1a from whole hippocampus tissue extract