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Fig. 4 | Molecular Brain

Fig. 4

From: TRPC1 mediates slow excitatory synaptic transmission in hippocampal oriens/alveus interneurons

Fig. 4

Co-localization of mGluR1a with TRPC1 and TRPC4 in O/A INs. a. Confocal images of double-label immunofluorescence of TRPC1 (green) and mGluR1a (red) in stratum oriens/alveus of the CA1 region. Left: TRPC1 antibody labelled the cell body and dendrites of three O/A interneurons (arrowheads), as well as other neuronal processes in stratum oriens/alveus. Middle: mGluR1a antibody also labelled cell bodies and proximal dendrites of the same interneurons. The cell body marked by an arrow showed less labelling than the two marked by arrowheads. Right: Merged images showing co-localization of TRPC1 and mGluR1a in cell bodies and proximal dendrites of O/A INs. b. Higher magnification confocal images of boxed region in A showing co-localization of TRPC1 and mGluR1a in a proximal dendrite of the O/A IN. c. Confocal images showing double-label of TRPC4 (green) and mGluR1a (red) in stratum oriens/alveus of the CA1 region. Left: TRPC4 antibody labelled cell bodies of O/A INs (arrowheads). Middle: mGluR1a antibody labelled the same cells. Right: Merged images showing co-localization of TRPC4 and mGluR1a. Scale bars: A and C, 25 μm; B, 10 μm

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