Table 2 PCR primers sequences and expected product size
From: TRPC1 mediates slow excitatory synaptic transmission in hippocampal oriens/alveus interneurons
mRNA | Accessionnumber | Sense primer | Antisense primer | Product size |
|---|---|---|---|---|
TRPC1 | NM_053558 | 5′-AAGAGCAGAAG | 5′-GTGCTCTGCATC | 562 bp |
GACTGCGTAG-3′ | TTCTGTCG-3′ |  | ||
TRPC2 | NM_022638 | 5′-CCTTCGAGTCAT | 5′-CCTTGGTCTCCA | 449 bp |
CAAGGCTC-3′ | GATCTTCC-3′ |  | ||
TRPC3 | NM_021771 | 5′-CATCTTCATTGC | 5′-CCACTCTACATC | 622 bp |
TGCCTTCA-3’ | ACTGTCATCC-3’ |  | ||
5′-CCTGAGCGAAGT | 5′-CCACTCTACATC | 529 bp | ||
CACACTCCCAC-3′§ | ACTGTCATCC-3’ |  | ||
TRPC4 | NM_080396 | 5-ATGAGGAATCTG | 5′-TATGTCTCTCGG | 316 bp |
GTGAAGCG-3’ | AGGCAATG-3’ |  | ||
TRPC5 | NM_080898 | 5′-C(G,T)ATGTTTGG | 5′-TGCAGCCACATA | 374 bp |
GACATACAACG-3’ | TCT(C,T)TTGAC-3’ |  | ||
TRPC6 | NM_053559 | 5′-GTGCCAAGTCCA | 5′-CTGGGCCTGCAG | 314 bp |
AAGTCCCTGC-3’ | TACGTATC-3’ |  | ||
TRPC7 | XM_225159 | 5′-CCTGTACTCCTA | 5′-TGGTGACATTAT | 182 bp |
CTACCGAGGTGC-3’ | AAACGCCGTAC-3’ |  | ||
GFAP | NM_017009 | 5′-ACCTCTGCACGC | 5′-TCCAGCGACTCA | 557 bp |
CGCTCCTATG-3’ | ACCTTCCTCT-3’ |  | ||
5′-ATTCCGCGCCTC | 5′-TTCATCCGCCTC | 435 bp | ||
TCCCTGTCTC-3′§§ | CTGTCTGT-3′§§ |  |