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Fig. 6 | Molecular Brain

Fig. 6

From: Toll-like receptor 4 deficiency ameliorates β2-microglobulin induced age-related cognition decline due to neuroinflammation in mice

Fig. 6

TLR4 elimination decreased the apoptototic neuronal death and increased the neurotrophic factors following B2M treatment. a Representative images of TUNEL-stained sections in hippocampal CA1 region of mice. Data were obtained from three independent animals per group, and the results of a typical result are exhibited. Scale bar = 100 μm. (n = 3/subgroup) (b) Quantitative analysis of the apoptosis index in hippocampal CA1 region. c-e Western blot analysis of apoptosis-related proteins cleaved-caspase-3, Bax and Bcl-2 in vivo experiment, β-actin was used as an internal control (n = 6/subgroup). f-h Western blot analysis of NGF and BDNF of hippocampus in vivo experiment, β-actin was used as an internal control (n = 6/subgroup). The data were analyzed using two-way ANOVAs used Tukey’s multiple comparisons test. Values are presented as the means ± SD. Significant differences are expressed as follows: *p < 0.05 compared B2M group vs. Veh group in WT and TLR4-KO mice, according to the two-way ANOVA; #p < 0.05 compared B2M group in TLR4-KO mice vs. WT mice, according to the two-way ANOVA. WT = wild type C57BL/6, TLR4-KO = TLR4 knockout

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