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Fig. 1 | Molecular Brain

Fig. 1

From: Unveiling synapse pathology in spinal bulbar muscular atrophy by genome-wide transcriptome analysis of purified motor neurons derived from disease specific iPSCs

Fig. 1

Establishment of iPSCs from SBMA patients and controls. a Immunocytochemical analysis of YFE-19 and SBMA3E-10 iPSCs for pluripotent markers OCT4 (green) and NANOG (red). Scale bar, 100 μm. The established iPSC clones were positive for OCT4 and NANOG. b Quantitative RT-PCR analysis of the expression of pluripotent makers OCT4 and NANOG in the established iPSC clones. Data are normalized to β-ACTIN and presented as the relative expressions to KhES1 (hESC). All established iPSC clones expressed OCT4 and NANOG. c Hematoxylin and eosin staining of teratomas derived from YFE-19 and SBMA3E-10 iPSCs. Scale bar, 200 μm. The established iPSC clones were able to differentiate into 3 germ layers. d Karyotype analysis of YFE-19 and SBMA3E-10 iPSCs via G-banding analysis. The established iPSC clones showed normal karyotypes, 46, XY. See also Additional file 1: Figures S1 and S2, Additional file 2

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