Fig. 3

ECV-mediated Aβ transfer is associated with inflammasome protein changes in NPC. HBMEC were treated, and ECVs were isolated as in Fig. 2, followed by exposure to NPCs for 24 h. a Representative images of NLRP3 immunoreactivity (red) in NPCs by confocal microscopy. Nuclei are stained with DAPI (blue). Scale bar: 20 μm. b Quantification of total and c nuclear NLRP3 from the confocal images. d Representative images of ASC immunoreactivity (red) in NPCs by confocal microscopy. Nuclei are stained with DAPI (blue). Scale bar: 20 μm. e Quantification of total and f nuclear ASC from the confocal images. Values are mean ± SEM, n = 3–7 (total); n = 47–86 (nuclear). Statistically significant at *p < 0.05, **p < 0.01, ***p < 0.001, or ****p < 0.0001