Nicotinamide attenuates the decrease in dendritic spine density in hippocampal primary neurons from 5xFAD mice, an Alzheimer’s disease animal model

Table 2 Hippocampal biomarkers that were significantly different (q < 0.05) both in 8 and 12 months

Metabolites	Regulation^a	8 M		12 M		Mass	RT	Ion mode	Fragment ion (m/z)
Metabolites	Regulation^a	q-value	p-value	q-value	p-value	(m/z)	(min)	Ion mode	Fragment ion (m/z)
Nicotinamide^b	down	0.00161	0.00044	0.00161	0.00044	123.0554	0.97	POS	80.0502, 78.0339, 96.0439
LysoPC (16:0)^b	up	1.62E-05	1.52E-06	1.62E-05	1.52E-06	496.3402	12.14	POS	184.0735, 104.1076
LysoPC (18:0)^b	up	5.07E-07	1.09E-08	5.07E-07	1.09E-08	524.3719	12.61	POS	184.0739, 104.1074
Phosphatidic acid^c	up	2.01E-05	2.22E-06	2.01E-05	2.22E-06	487.2795	12.61	POS	445.2678
AMP^a	down	4.10E-06	8.02E-07	4.10E-06	8.02E-07	346.0545	0.97	NEG	96.9692, 134.0466, 150.9503
LysoPE (16:0)^c	up	1.67E-07	2.10E-08	1.67E-07	2.10E-08	452.2761	11.98	NEG	255.2324, 112.9855, 196.0417
LysoPE (16:0)^a	up	5.52E-08	5.39E-09	5.52E-08	5.39E-09	452.2772	12.1	NEG	255.2326, 122.9857, 196.0413
LysoPE (18:0)^c	up	1.37E-06	2.20E-07	1.37E-06	2.20E-07	480.3085	12.13	NEG	255.2322

^aUp- or down- regulation in 5xFAD vs. WT. ^b identified metabolite compared with authentic standard. ^cputative metabolites compared with database. RT Retention time, CE Colision energy

ISSN: 1756-6606