Fig. 1
From: Regulation of behavioral response to stress by microRNA-690
Overexpression of miR-690 in the mPFC led to stress-resilient behaviors following restraint stress in mice. a Volcano plot representing the differentially expressed miRNAs in the mPFC of Fkbp5 KO mice, satisfying the criterion of P value < 0.05. Significantly altered miRNAs are indicated by blue (downregulated) and red (upregulated) dots. b The expression of mmu-miR-690 in the mPFC of mice subjected to the restraint stress. WT control mice (WT_CT, n = 9); stressed WT mice (WT_ST, n = 6); stressed Fkbp5 KO mice (KO_ST, n = 6). One-way ANOVA (F [2, 18] = 10.65, P = 9.00 × 10−4); Fisher’s LSD (**P < 0.01, ***P < 0.001). c AAV-miR-690 and AAV-control vector design; ITR inverted terminal repeat, CMV cytomegalovirus; GFP green fluorescent protein, SV40 simian virus 40. d Schematic timeline of experimental procedures and behavioral tests. Restraint stress (above) and conditioned fear stress combined with single-prolonged stress (CF + SPS) (below). e–g Influence of overexpression of miR-690 in the mPFC on mouse behavior following restraint stress. Results of sucrose preference test (e). Non-stressed control mice (n = 5); stressed control mice (n = 8); non-stressed miR-690 mice (n = 5); stressed miR-690 mice (n = 8). Two-way ANOVA (stress × virus, F [1, 22] = 10.39, P = 3.90 × 10–3); Fisher’s LSD (**P < 0.01). Results of forced swim test (f). Non-stressed control mice (n = 8); stressed control mice (n = 8); non-stressed miR-690 mice (n = 8); stressed miR-690 mice (n = 8). Two-way ANOVA (stress, F [1, 28] = 9.97, P = 3.80 × 10–3); Fisher’s LSD (*P < 0.05, **P < 0.01). Results of novel object recognition test (g). Non-stressed control mice (n = 6); stressed control mice (n = 8); non-stressed miR-690 mice (n = 6); stressed miR-690 mice (n = 7). Two-way ANOVA (stress × virus, F [1, 23] = 5.81, P = 2.44 × 10−2); Fisher’s LSD (*P < 0.05, **P < 0.01). h Effects of CF + SPS and miR-690 on elevated plus maze test. Non-stressed control mice (n = 5); stressed control mice (n = 4); non-stressed miR-690 mice (n = 4); stressed miR-690 mice (n = 4). Two-way ANOVA (stress, F [1, 13] = 20.45, P = 6.0 × 10−4); Fisher’s LSD (*P < 0.05, **P < 0.01, ***P < 0.001). In all data, black dots and red dots indicate non-stressed mice and stressed mice, respectively. Bars represent group mean and error bars represent SEM