Fig. 2

a ZNF804A protein physically interacts with LGALS1, FEZ1, and RPSA. (b, i) Zfp804a shRNA or a scramble shRNA are co-transfected with constructs expressing FLAG tag (b, c), LGALS1 (d, e), FEZ1 (f, g) or RPSA (h, i) separately into primary mouse cortical neurons. The transfected neurons are stained with GFP (green). Bar = 50 µm. Zfp804a knockdown in differentiating neurons affects number of branches (j), total dendritic length (k), number of primary branches (l), and number of non-primary branches (m). The Sholl analysis indicates the complexity of the neurite outgrowth (n), and illustrates representative co-transfected neurons with concentric circles (o–v). The interval between adjacent consecutive circles is 10 μm (n = 70)