Fig. 4

ZNF804A and empty GFP overexpression vectors were co-transfected with vectors expressing FLAG tag (a, b), LGALS1 (c, d), FEZ1 (e, f) or RPSA (g, h) separately into primary neurons. The transfected neurons were stained with GFP (green). Bar = 50 µm. ZNF804A overexpression in differentiating neurons leads to reduced number of branches (i) and total dendritic length (j), number of primary branches (k), and number of non-primary branches (l). FEZ1 restored deficits of both neurite number and neurite length. FEZ1 and RPSA increase the primary (k) and non-primary (l) neurite number that are decreased by ZNF804A overexpression. The Sholl analysis shows the complexity of the neurite outgrowth (m), and illustrates representative co-transfected neurons with concentric circles (n–u). The interval between adjacent consecutive circles is 10 μm (n = 70)