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Fig. 1 | Molecular Brain

Fig. 1

From: Conditional Pten knockout in parvalbumin- or somatostatin-positive neurons sufficiently leads to autism-related behavioral phenotypes

Fig. 1

PV-Pten-KO and Sst-Pten-KO mice displayed social deficits in the modified three-chamber social test. a Schematic diagrams of the modified three-chamber social test. b All three genotypes of PV-Pten mice showed significant social preference between objects and mice (social partners) (WT: n = 26, W = 325, p < 0.0001, Wilcoxon test; Het: n = 17, t(16) = 5.065, p = 0.0001, paired t-test; KO: n = 21, W = 151, p = 0.0071, Wilcoxon test). There is no significant difference in preference index for social stimulus among these three genotypes of PV-Pten mice (F(2,61) = 0.213, p = 0.809, one-way ANOVA). c Only PV-Pten-WT mice displayed significant preference between familiar and novel mice in the social novelty test (WT: n = 26, t(25) = 6.142, p < 0.0001; Het: n = 17, t(16) = 0.7481, p = 0.4652; KO: n = 21, t(20) = 0.9664, p = 0.3454; Paired t-test). The preference index for social novelty significantly reduced in PV-Pten-Het and PV-Pten-KO mice compared with PV-Pten-WT mice (F(2,61) = 7.402, p = 0.0011, one-way ANOVA; Het vs. WT: p = 0.0427, KO vs. WT: p = 0.0011, Tukey’s post hoc test). d All three genotypes of Sst-Pten mice showed significant social preference between objects and social partners (WT: n = 22, t(21) = 4.570, p = 0.0002, paired t-test; Het: n = 29, W = 221.0, p = 0.0157, Wilcoxon test; KO: n = 24, W = 254.0, p < 0.0001; Wilcoxon test). The preference index for social stimulus reduced slightly in Sst-Pten-Het mice and increased in Sst-Pten-KO mice, but none of these changes reached statistical significance in post hoc comparisons (p = 0.0008, Kruskal–Wallis test; Het vs. WT: p = 0.2287, KO vs. WT: p = 0.0855, Dunn’s post hoc test). e Sst-Pten-Het and Sst-Pten-KO mice displayed deficits in the social novelty test (WT: n = 22, t(21) = 5.269, p < 0.0001, paired t-test; Het: n = 29, W = 99, p = 0.2941, Wilcoxon test; KO: n = 24, t(23) = 1.775, p = 0.0891, paired t-test). The preference index for social novelty significantly reduced in Sst-Pten-Het and Sst-Pten-KO mice (p = 0.0311, Kruskal–Wallis test; Het vs. WT: p = 0.0442, KO vs. WT: p = 0.0384, Dunn’s post hoc test). The preference index was calculated as described in the methods. Circles represent data from individual animals, and bar graphs indicate mean + SEM. *: significant; n.s.: not significant

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