Fig. 4

Impaired motor function in PV-Pten-KO and Sst-Pten-KO mice. a The schematic diagram of the rotarod test. b PV-Pten-Het and PV-Pten-KO mice showed a shorter latency to fall off the rotarod than PV-Pten-WT mice over the 6-trials training (WT: n = 14, Het: n = 17, KO: n = 14; p < 0.0001, genotype effect, two-way rmANOVA; Het vs. WT: p < 0.0001, KO vs. WT: p < 0.0001, Het vs. KO: p < 0.0001, Tukey’s post hoc test). c The learning index (T6/T1) was significantly reduced in PV-Pten-KO mice (F_(2,44) = 12.435, p < 0.001, one-way ANOVA; KO vs. WT: p < 0.001, Tukey’s post hoc test). d Sst-Pten-KO mice showed a shorter latency to fall off the rotarod than Sst-Pten-WT mice over the 6-trials training (WT: n = 10, Het: n = 16, KO: n = 17; p = 0.0005, genotype effect, two-way rmANOVA; KO vs. WT: p = 0.0004, Tukey’s post hoc test). e The learning index (T6/T1) was significantly reduced in Sst-Pten-KO mice (F_(2,40) = 7.372, p = 0.0019, one-way ANOVA; KO vs. WT: p = 0.0065, Tukey’s post hoc test). f The schematic diagram of the marble burying test. g PV-Pten-KO mice buried significantly fewer marbles compared to PV-Pten-WT mice (WT: n = 22, Het: n = 18, KO: n = 16; p < 0.001, Kruskal–Wallis test; KO vs. WT, p = 0.001, Dunn’s post hoc test). h There was no difference in the number of marbles buried among the three genotypes of Sst-Pten mice (WT: n = 7, Het: n = 8, KO: n = 10; p = 0.406, Kruskal–Wallis test). In c, e, g and h, circles represent data from individual animals, and bar graphs indicate mean + SEM. In b and d, data are represented as mean ± SEM. *: significant; n.s.: not significant