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Fig. 3 | Molecular Brain

Fig. 3

From: Mice with cleavage-resistant N-cadherin exhibit synapse anomaly in the hippocampus and outperformance in spatial learning tasks

Fig. 3

Protein profiles of GD mouse brain is normal but for the absence of CTF1. a Representative immunoblot of endogenous proteins related to glutamatergic synapse and/or N-cadherin in the total brain lysate and a synaptosomal fraction from GD and WT mice. No recognizable difference was found in syntaxin 6, PSD95, synaptophysin, full-length N-cadherin, phospho-GluA1, GluA1, GluA2, GLT-1, phospho-AKT, AKT, α-tubulin). b Immunoblot detection of endogenous N-cadherin with an antibody against a C-terminal region in the total lysates of primary cultured cerebrocortical neurons from WT and GD mice. While the full-length form (FL, 130 kDa) was detected both in WT and GD samples, the cytoplasmic fragment CTF1 (45 kDa) was detected only in those from WT, but not in those from GD mice (left). Similar results were obtained from the synaptosomal fraction (right). Non-specific bands appeared around 50 kDa (*) and above

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