Fig. 1

Systematic workflow of three phases in the study. In the discovery phase, 8-plex iTRAQ was conducted, and bioinformatic analysis was performed. In the verification phase, PRM was applied to verify the outcomes of quantitative proteomic analysis. In the validation phase, a protein of interest (transthyretin) was identified, and thyroxine was used to detect the effects. Behavioral outcomes and histopathology were observed, and conclusions were made