Fig. 5

Effects of GluN2C/2D antagonists to the synaptic plasticity in the ACC. a Left: LTP was induced in pyramidal neurons in adult ACC (black squares, n = 5 neurons/ 3 mice) by the pairing training protocol (indicated by an arrow). Pretreated slices with 3 μM UBP145 didn’t affect the induction of LTP (red circles, n = 4 neurons/ 3 mice). Sample traces of evoked EPSCs during baseline (1) and 30 min after the induction stimulus (2) are showed on the top; Right: Summary of the effect of the UBP145 on the postsynaptic LTP. Data within the last five minutes of the baseline and 30 min after the induction are averaged. b Top: pre-LTP was induced in pyramidal neurons in the ACC of adult mice (black squares, n = 4 neurons/ 3 mice) by giving 240 pulses at 2 Hz (indicated by an arrow). Pretreated slices with 3 μM UBP145 did not affect the induction of pre-LTP (red circles, n = 4 neurons/ 3 mice). Sample traces of evoked EPSCs with paired-pulse stimulation at 50 ms at a holding membrane potential of -70 mV during baseline (1) and 30 min after the induction stimulus (2) are showed on the top; Bottom: PPR values for the control (black squares) and UBP145 group (red circles). c LTD was induced in pyramidal neurons in adult ACC (black squares, n = 5 neurons/ 3 mice) by the pairing training protocol (300 pulses at 1 Hz while holding at -45 mV indicated by an arrow). Pretreated slices with 3 μM UBP145 did not affect the induction of LTD (red circles, n = 4 neurons/ 3 mice). Sample traces of evoked EPSCs during baseline (1) and 30 min after the induction stimulus (2) are showed on the top. **p < 0.01, ***p < 0.001. Error bars represent SEM