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Fig. 4 | Molecular Brain

Fig. 4

From: GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter

Fig. 4

PV ( +) neuron-preferential transduction by the mGAD65 promoter. a The expression cassette of AAV-PHP.B expressing GFP under the control of the mGAD65 promoter. b Immunohistochemistry of the motor cortices from wild-type mice 3 weeks after the viral injection. Brain sections were immunostained for PV (light blue) and somatostatin (SST) (magenta). Blue arrowheads indicate GFP-expressing PV ( +) neurons, and magenta arrowheads show GFP-expressing SST ( +) neurons. Scale bar; 20 μm. c Bar and circle graphs showing proportions of PV ( +), SST ( +), and other cell types among GFP-expressing cells. We observed 2,884 GFP ( +) cells (n = 5 sections from 5 mice). d Floating bar and circle graphs showing natural ratios of PV ( +), SST ( +), and other cell types to all GABAergic interneurons in the motor cortex of non-injected control mice. The proportions were determined by immunohistochemistry using VGAT-tdTomato mice. We examined 2,027 tdTomato ( +) cells (n = 4 sections from 4 mice). e Floating bar graph showing the proportions of GFP-expressing cells in PV ( +) or SST ( +) neurons. We found 733 GFP-expressing cells in 802 PV ( +) cells (91.2 ± 1.6%) and 134 GFP-expressing cells in 264 SST ( +) cells (50.7 ± 4.5%) (n = 5 sections from 5 mice). ce Floating bars indicate upper, median, and lower quartiles (boxes) and value from each mouse (dots). Note that the majority (about 60%) of transduced cells were PV ( +) neurons (c). PV ( +) neurons accounted for approximately 40% of all interneurons in the motor cortex (d). More than 90% of PV ( +) neurons were transduced in contrast to approximately 50% of SST ( +) neurons that were not transduced (e)

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