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Fig. 7 | Molecular Brain

Fig. 7

From: GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter

Fig. 7

ad Assessment of Ca2+ activities in GABAergic inhibitory neurons in the motor cortex using the mGAD65 promoter. Left panels show the averaged confocal images (averaging hundreds of frames) of G-CaMP signals (green) and single tdTomato images (magenta) of the same location in the M1 or M2 region of the motor cortex in the acute brain slices. Regions of interest (ROIs) were placed in somas (a, b; yellow), an axon (b, blue), and puncta (cd; yellow) of the G-CaMP ( +) and tdTomato ( +) structures for Ca2+ signal analysis. Right panels represent traces of spontaneous Ca2+ signal changes calculated from the ROIs depicted in the left panels. e, f Left panels show individual traces of relative Ca2+ signals in several somas of GABAergic neurons in individual fields of view. Bath application of 10 µM glutamate (Glu, e) or high K+ solution (f) induced an increase in Ca2+ signals. The right panels (box plots) show open circles indicating individual data points of the Ca2+ signal peak values. The horizontal line and the box represent the median value and the interquartile range, respectively. The error bars indicate one standard deviation above and below the mean value (filled circle)

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