Fig. 3

Co-immunostaining of Olig2 and CC1 in adult mouse CNS. a Sagittal overview of Olig2 and CC1 expression pattern in the mouse brain. b–m High magnification images showing the expression relationship between Olig2 and CC1 in brain regions of interest. Most of the CC1⁺ cells have Olig2 immuno signal as indicated by white arrowheads. On the other hand, white arrows indicate Olig2⁺ cells without CC1. The proportion of CC1⁺ and Olig2⁺ cells is about 50% in the STR and HIP, and higher than 80% in other brain regions. n Coronal overview of the mouse SC, showing co-immunostaining of Olig2 and CC1. o High magnification image displaying the co-immunostaining of Olig2 and CC1 in the SCe. As in the brain regions, the majority of the CC1⁺ cells are positive for Olig2 (white arrowheads), quantified data showing about 77.0% of Olig2⁺ cells are CC1⁺ (white arrows). Scale bars: 1000 μm (a), 50 μm (b–m, o), 500 μm (n)