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Fig. 1 | Molecular Brain

Fig. 1

From: PDK1 is a negative regulator of axon regeneration

Fig. 1

PDK1 is downregulated in vitro after axonal injury in cultured embryonic DRG neurons. a Western blot analysis of embryonic DRG neuron cultures with (+) or without (−) axotomy. Protein lysates were prepared 6 h after axotomy. p-PDK1 (Ser241) is PDK1 phosphorylated at serine 241. b Relative intensity of PDK1 and p-PDK1 (Ser241) Western bands from (a) (Mean ± S.E.M.; n = 3, **p < 0.01 from a t-test). c Relative mRNA levels measured using quantitative RT-PCR from cultured embryonic DRG neurons with (+) or without (−) axotomy (n = 3; ns from a t-test). d Western blot analysis of protein lysates from the cell bodies and axons prepared separately with (+) or without (−) axotomy. Numbers indicate normalized relative intensities

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