Fig. 2

PDK1 is upregulated in vivo at sites adjacent to the injury in mouse sciatic nerves. a Western blot analysis of protein lysates from mouse sciatic nerves with (+) or without (−) injury. Protein lysates were prepared 24 h after axotomy. b Relative intensity of PDK1 and p-PDK1 (Ser241) Western bands from (a) (Mean ± S.E.M.; n = 5, ns from t-test). c Representative immunohistochemistry of longitudinal sections of mouse sciatic nerves with (+) or without (−) injury. P, proximal to the DRG tissues, D, distal to the nerve terminus. Scale bar, 500 μm. Relative normalized intensity of PDK1 is plotted with x-axis of distance and y-axis of relative ratio. d Representative immunohistochemistry of mouse L4 DRG tissue with (+) or without (−) injury. Scale bar, 50 μm