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Fig. 3 | Molecular Brain

Fig. 3

From: Phosphorylation of GAP-43 T172 is a molecular marker of growing axons in a wide range of mammals including primates

Fig. 3

Axon regeneration of the injured sciatic nerve in adult mice is tightly associated with T172 phosphorylation of GAP-43. a–c Immunofluorescent studies of the sciatic nerves in longitudinal sections on days 1 (a, b) and 3 (c) after the crush using pT172, SCG-10, and Tuj-1 (neuron-specific β3 tubulin) Abs. SCG-10 was used as a positive control for axon regeneration. Both sections of (a) and (b) were adjacent with each other. d pT172 immunoreactivity was not detected in the contralateral intact side against (a, b). e Regeneration index [12, 13, 21] of pT172, compared to that of SCG10. The regeneration index on day 3 was higher than that on day 1. n = 3 (for each group). ***p = 0.0005 by one-way ANOVA with Bonferroni test. f The immunoreactivity of pT172 and pS96 on the sciatic nerve after the crush on day 1. White arrowheads: injury point; black arrowheads: the farthest point of positive immunoreactivity. Scale bar: 500 µm (a–d, f). Z-stack average-intensity projection images are shown (a–d, f). g Ligation of the sciatic nerves. In samples 6 h after ligation, pT172Ab immunoreactivity was stronger than in the proximal region than that in the distal one to the ligation site. Arrow: the ligation point. Scale bar: 500 μm. DAB staining was used for detection [12, 13, 17]. Ligation experiments were performed by method of Cavalli et al. [63]. See also Additional file 5: Fig. S4

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