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Fig. 4 | Molecular Brain

Fig. 4

From: Phosphorylation of GAP-43 T172 is a molecular marker of growing axons in a wide range of mammals including primates

Fig. 4

pT172 recognized growing axons in the ferret embryonic brain. a The pT172 site (*) and its surrounding amino acid sequences in GAP-43 are conserved from rodents to primates. Human (H. sapiens; 238 aa; T181), cynomolgus monkey (Macaca fuscata; 238 aa; T181), common marmoset (Callithrix jacchus; 177–186, 238 aa; T181), ferret (Mustela putorius furo; 242 aa; T185), rat (Rattus norvegicus; 226 aa; T172), and mouse (Mus musculus; 227 aa; T172). Note that the amino acid sequences of T172 and the following P173 in mouse are completely conserved in other mammals. b Immunohistochemistry (DAB staining) using pT172Ab in E40 ferret brain. Scale bar: 1 mm. c Patterns of immunoreactivity with pT172Ab in developing ferret cortices (P0, P10, P16, adult; duplicate samples). GAPDH and β-actin: positive controls. pT172 pAb was diluted to 1:1,000. Arrowheads: the predicted molecular mass. In the adult brain lysate, frontal lobes were used

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Molecular Brain

ISSN: 1756-6606