Fig. 2
From: Caveolin-1 deficiency impairs synaptic transmission in hippocampal neurons
Synaptic vesicle exocytosis is slowed in Cav1-KD neurons. a Representative images of western blotting. Neurons transfected with shRNA-Cav1 were lysed, after which proteins in lysates were resolved by SDS-PAGE and transferred to PVDF membranes. Membranes were subsequently incubated with anti-Cav1 antibody and anti-β-actin antibody. b Cav1 expression levels in control and Cav1-KD neurons. Intensities of Cav1 bands expressed relative to those of β-actin are presented as means ± SEMs. ([Cav1Con] = 100.00 ± 6.8; [Cav1KD] = 22.45 ± 3.2, n = 4). c Representative images of Physin-pH at synaptic boutons in Control, Cav1-KD, and Cav1-Rescue (Cav1-Res) neurons under basal conditions (top), with stimulation at 10 Hz for 10 s (100 APs; middle), and following application of NH4Cl (bottom). Scale bar: 10 μm. d Representative trace of Physin-pH responses to 100 APs in Control (black), Cav1-KD (red), and Cav1-Res (green) neurons. e Quantification of synaptic transmission, expressed as means ± SEMs in Control (0.15 ± 0.02; n = 14 cells), Cav1-KD (0.07 ± 0.01; n = 14 cells) and Cav1-Res (0.14 ± 0.02; n = 7 cells) neurons (***p < 0.001, **p < 0.01). f and g Cumulative frequency of exocytosis from single synaptic bouton analyses of Control, Cav1-KD, and Cav1-Res neurons