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Fig. 5 | Molecular Brain

Fig. 5

From: Caveolin-1 deficiency impairs synaptic transmission in hippocampal neurons

Fig. 5

Expression of the Cav1 palmitoylation mutant, Cav1 tri-mut, fails to rescue synaptic transmission and retrieval defects in neurons. a Representative traces of exocytosis from Control (black) and Cav1 tri-mut (red)–expressing neurons. Neurons were transfected with Physin-pH with or without shRNA-Cav1 and Cav1 tri-mut and stimulated with 100 APs at 10 Hz. b Quantification of synaptic transmission expressed as means ± SEMs in Control (black; 0.15 ± 0.02; n = 14 cells) and Cav1 tri-mut (red; 0.06 ± 0.01; n = 11 cells) neurons. c Representative traces of Physin-pH responses to 100 APs in Control (black) and Cav1 tri-mut (red) neurons. d Quantification of post-stimulus endocytic time constants, expressed as means ± SEMs in Control (τendo, 13.71 ± 0.92; n = 14 cells) and Cav1 tri-mut (τendo, 20.06 ± 2.79; n = 8 cells) neurons. e Representative normalized traces of Physin-pH exocytosis in Control (black) and Cav1 tri-mut (red) neurons. f Quantification of time constants of exocytosis expressed as means ± SEMs in Control (τexo = 34.61 ± 4.23; n = 12 cells) and Cav1-KD (τexo = 62.56 ± 7.01; n = 9 cells) neurons. g Representative traces of Physin-pH responses to 2000 APs at 10 Hz in the presence of bafilomycin A1 (BAF) in control and Cav1 tri-mut neurons. h Quantification of recycling vesicle pool sizes expressed as means ± SEMs. in Control (0.62 ± 0.04; n = 12 cells) and Cav1 tri-mut (0.56 ± 0.07; n = 9 cells) neurons. i Representative images of primary cultured hippocampal neurons exogenously co-expressing Cav1 tri-mut and synaptophysin-pH. j Correlation between Cav1 tri-mut and synaptophysin-pH expression levels (***p < 0.001, **p < 0.01, *p < 0.05). Scale bar: 10 μm (up) and 2 μm (bottom)

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