Fig. 1
From: The Ser19Stop single nucleotide polymorphism (SNP) of human PHYHIPL affects the cerebellum in mice
Generation of PHY2 Ser19Stop knock-in mice. a Alignment of mouse PHY1 (top) and PHY2 (bottom) protein sequences. Identical amino acids are shown in the middle. The dashed line represents the Fibronectin type-III domain. b Tissue distribution of PHY2 expression in P56 mice. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control. c The Ser19Stop SNP of human PHY2 registered in the dbSNP (rs7907875). d A portion of the coding region in the mouse PHY2 gene was replaced with Ser19Stop SNP. e Immunoblot analysis of P21 cerebellum of wild-type (PHY2+/+), heterozygous (PHY2+/S19X), and homozygous (PHY2S19X/S19X) mice. Protein lysates were immunoblotted with anti-PHY2 and anti-actin antibodies