Skip to main content
Fig. 1 | Molecular Brain

Fig. 1

From: The C. elegans homolog of human panic-disorder risk gene TMEM132D orchestrates neuronal morphogenesis through the WAVE-regulatory complex

Fig. 1

C. elegans TMEM-132 specifically localizes to the nervous system. a Schematic of human TMEM132D and C. elegans TMEM-132 protein domains. The Pfam16070 conserved domain (green) characterizes both proteins, with additional transmembrane domains (TM, blue) and C-terminal regions (red) used for yeast-two-hybrid assays. b Exemplar confocal fluorescence image showing expression of tmem-132 promoter-driven GFP in the nervous system. Inset, exemplar confocal fluorescence image showing expression of tmem-132 promoter-driven GFP specifically in the PDE neuron. c Exemplar confocal fluorescence image showing the puncta pattern of tmem-132 promoter-driven TMEM-132::GFP expression in the ventral nerve cord. d Exemplar confocal fluorescence image showing the puncta pattern of tmem-132 promoter-driven TMEM-132::GFP expression in the nerve ring. Scale bar: 50 µm. e Schematic of tmem-132 gene structure showing positions of alleles generated by CRISPR-mediated editing including deletion alleles dma317, dma318, dma319 and point mutation dma313 that converts proline 784 to threonine. f Multiple sequence alignment (generated by ClustalOmega and visualized by Jalview) of TMEM132 protein family from indicated metazoan species showing high levels of amino acid sequence conservation around the P784 position. P784T is one of the non-synonymous variants identified as risk alleles for panic disorder and anxiety

Back to article page