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Fig. 5 | Molecular Brain

Fig. 5

From: The C. elegans homolog of human panic-disorder risk gene TMEM132D orchestrates neuronal morphogenesis through the WAVE-regulatory complex

Fig. 5

Human TMEM132D interacts with Nap/Sra via C-terminal domains. a Exemplar Western blot showing CoIP of various human TMEM132D (V5-tagged) mutants and NAP1 (GFP-tagged). Expression was driven by constitutive CMV promoters in HEK293 cells and lysates were used for GFP-trap and blot with antibodies against GFP and V5. b Exemplar Western blot showing CoIP of various human TMEM132D (V5-tagged) mutants and SRA1 (GFP-tagged). 60 a.a. D, TMEM132D with deletion of the last 60 a.a. sequence. 120 a.a. D, TMEM132D with deletion of the last 120 a.a. sequence. WIRS, TMEM132D with the WIRS-like motif (KFTTFTAV) mutated to alanine residues. ECD, TMEM132D with only extracellular domain and transmembrane domain. Results represent three independently repeated experiments. c Schematic showing human TMEM132D or C. elegans TMEM-132 with various domain genetic deletions and a summary of their interaction with Nap homologs (human NAP1 and C. elegans GEX-3) from both CoIP and Y2H assays

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