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Fig. 7 | Molecular Brain

Fig. 7

From: The C. elegans homolog of human panic-disorder risk gene TMEM132D orchestrates neuronal morphogenesis through the WAVE-regulatory complex

Fig. 7

TMEM132D decreases abundance of selective WAVE components. a Exemplar epifluorescence images showing effects of TMEM132D overexpression on abundance of GFP-tagged WAVE components in HEK293 cells. Expression constructs encoding TMEM132D and individual components of WRCs or GFP only control were co-transfected for expression and imaging at 48 h post transfection, followed by quantification of percentage of GFP + cells as shown in Fig. 7d. b Exemplar Western blot showing decreased abundance of ABI1 but not NAP1 by co-expression with V5-tagged TMEM132D. Comparable abundance of V5 and H3 controls effects of TMEM132D expression levels and sample loading. c Exemplar Western blot showing decreased abundance of LifeAct::GFP reporter by co-expression with V5-tagged TMEM132D, under serum-containing and serum-free media conditions. Comparable abundance of V5, pan-Actin and H3 controls effects of TMEM132D expression levels, monomeric Actin and sample loading respectively. d Quantification of percentage of GFP + cells under indicated co-transfection conditions. e Exemplar micrographic images showing motile recovery of HEK293 cells stably expressing control or TMEM132D after line wounding. f Quantification of cell-free line width indicating wound recovery in HEK293 cells stably expressing control or TMEM132D 24 and 48 h after line wounding. Scale bars: 10 µm. **Indicates P < 0.01 (repeated in at least three independent experiments)

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Molecular Brain

ISSN: 1756-6606