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Fig. 1 | Molecular Brain

Fig. 1

From: α1A-adrenaline receptors in dorsal horn inhibitory neurons have an inhibitory role in the regulation of chloroquine-induced itch in mice

Fig. 1

Knockout of α1A-ARs in inhibitory interneurons enhances scratching behavior evoked by chloroquine. a Scratching behaviors induced by intradermal injection of compound 48/80 (50 μg/50 μl) or chloroquine (200 μg/50 μl) in Adra1aflox/flox mice or Vgat-Cre;Adra1aflox/flox mice. *P < 0.05. b RNAscope in situ hybridization for mRNAs of Adra1a (red) and Slc32a1 (green) in the SDH of Adra1aflox/flox mice or Vgat-Cre;Adra1aflox/flox mice. 4,6-diamidino-2-phenylindole (DAPI) to mark nuclei is blue. Arrowheads and arrows indicate Adra1a+ Slc32a1+ cells and Adra1a Slc32a1+ cells, respectively. Scale bar, 20 μm. c Percentage of Adra1a+ Slc32a1+ cells per total Slc32a1+ cells tested in the SDH of Adra1aflox/flox mice or Vgat-Cre;Adra1aflox/flox mice. **P < 0.005. d Schematic illustration of transduction strategy of SaCas9 and guide RNA (sgAdra1a and sgRosa (control)) expression in SDH inhibitory neurons. e Effect of genome editing on scratching behaviors induced by intradermal injection of compound 48/80 (50 μg/50 μl) or chloroquine (200 μg/50 μl). *P < 0.05. Data show the mean ± SEM

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