Fig. 1
Oxytocin ameliorates impaired social interaction in POGZWT/Q1038R mice. a Time course of the reciprocal social interaction test. The test was performed 30 min after intranasal administration of oxytocin (200 μg/kg) or saline. The duration of sniffing was measured during the test. i.n. intranasally, WT wild-type. b Time spent sniffing in the reciprocal social interaction test after oxytocin treatment (each n = 10). c qPCR analysis of the expression levels of OXTR and AVPR1a mRNA in the brain (each n = 3). OXTR, oxytocin receptor; AVPR1a, vasopressin receptor 1A. d Representative western blotting and quantification of decreased OXTR in POGZWT/Q1038R mice. GAPDH was used as loading control (each n = 3). Values were normalized to the expression levels of GAPDH. Uncropped western blot images are shown in Additional file 2: Fig. S1. e Representative image of OXT (green) and Hoechst 33258 (blue) immunofluorescence in the PVN and stereological counts of OXT-expressing cells in the PVN region of WT and POGZWT/Q1038R mice (each n = 3; scale bar, 300 μm). f Schematic diagram of the position of the qPCR amplicons by each primer set in the mouse OXTR promoter region. TSS translation start site. g ChIP assay coupled with qPCR for the quantification of DNA fragments precipitated by an anti-POGZ antibody (n = 1 mice). The results are presented as percentage of the input DNA. n.d. not detected. Data are presented as the mean ± SEM. Statistical significance was analyzed by two-way ANOVA, followed by Bonferroni Dunn post hoc tests (b, F1, 36 = 11.67) and Student’s t-test (c–e). *P < 0.05, **P < 0.01