Fig. 5

Live cell imaging of mitochondrial membrane potential in dopaminergic neurons derived from TH-GFP iPSCs. a Live cell imaging of dopaminergic and non-dopaminergic neurons derived from control and PRKN-mutated TH-GFP iPSCs. Arrowheads indicate weak TMRM signals in GFP-positive dopaminergic neurons. Arrows indicate strong TMRM signals in GFP-negative non-dopaminergic neurons. “PRKN” represents PRKN-mutated patient. Scale bar, 5 µm. b Quantitative analysis of the MFI of TMRM (left), Mitotracker DeepRed (center), and TMRM normalized to Mitotracker DeepRed (right) in control GFP-positive cells relative to control GFP-negative cells in fluorescence images (n = 20 images). The MFI was measured using the surrounding cytoplasmic area in ZEN software. Values are shown as the mean ± SEM. Statistical significance was evaluated using the unpaired two-tailed t-test. **P < 0.01, ****P < 0.0001. c Quantitative analysis of the MFI of TMRM (left), Mitotracker DeepRed (center), and TMRM normalized with Mitotracker DeepRed (right) in PRKN-mutated GFP-positive cells relative to PRKN-mutated GFP-negative cells in fluorescence images (n = 20 images). “PRKN” represents PRKN-mutated patient. Values are shown as the mean ± SEM. Statistical significance was evaluated using the unpaired two-tailed t-test. **P < 0.01. There were no significant differences in the MFI of Mitotracker and TMRM/Mitotracker between GFP-positive and -negative cells (b″; P = 0.1163, b‴; P = 0.0551)