Fig. 5

Effect of pre-treatment with Trolox in the Tg2576 mice. Aa Representative trace in the current-clamp recording of pre-treatment with Trolox for one hour (orange) and 1 week (purple) in Tg2576 in response to depolarizing current injection (200 pA with 1 s duration). Ab F-I curve (from 100 to 600 pA, 100 pA increment) obtained from GCs of Tg2576 mice treated with Trolox for 1 week (Tg + Trolox, purple, n = 8) was compared with the F-I curve obtained from Tg2576 GCs (green dashed line, n = 14) shown in Fig. 2Ab. *, P < 0.05, ***, P < 0.001. B Western blot analysis for Kv4.1 in DG of 8-week-old Tg2576 mice and those treated with Trolox (20 mg/kg) for 1 week. For a loading control, β-actin was used. Right, Kv4.1expression in Tg2576 was significantly increased after Trolox treatment. **, P < 0.01. Ca On day 4–5, the kinetics of freezing across the 5 min test in context A obtained from Tg2576 mice treated with Trolox for 1 week (Tg + Trolox, purple, n = 10) was compared with the that obtained from control Tg2576 GCs (green, n = 7) shown in Fig. 1Ba. Cb Percentage of freezing in context A (filled bar) and context B (open bar) during day 4 to 5. Tg2567 (green, n = 7) and Tg + Trolox (purple, n = 10) mice displayed equal amounts of freezing in both contexts A and B. Da On day 6 to 12, time course of the discrimination ratio in Tg + Trolox mice (purple, n = 10) was compared with that obtained from control Tg2576 mice shown in Fig. 1Ca (green, n = 7) mice. Statistical significance for each day was tested using unpaired t-test (*P < 0.05; **P < 0.01; ***P < 0.001). Db Percentage of freezing in context A (filled bar) and context B (open bar) for Tg2576 (green, n = 7) and Tg + Trolox (purple, n = 10) mice on day 11. Freezing levels were compared between the two contexts for each group. Tg2567 (green, n = 7) mice displayed equal amounts of freezing in both contexts A and B, while and Tg + Trolox (purple, n = 10) mice displayed more freezing in context A compared to context B