Fig. 2
From: Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cells
Palmitate impairs ER-phagy. a and b Immunoblotting analysis (a) and quantification (b) of ER-phagy receptors (FAM134B, RTN3, and CCPG1) in cells treated with 0.1 mM palmitate for the indicated times (FAM134B and RTN3; n = 4 per each group, CCPG1; n = 3 per each group). Data are mean ± SEM; FAM134B; **p = 0.0053, ***p = 0.0005 vs. control, CCPG1; *p = 0.0388, ***p = 0.0004 vs. control. c and d Cells transiently expressing mRFP-LC3 were treated with 0.1 mM palmitate for the indicated times in the presence or absence of Baf A1 (200 nM). Treated cells were stained for the ER marker KDEL. Representative micrographs (c) and quantification (d) of colocalization between mRFP-LC3 and KDEL (n = 13 per each group). Scale bar, 10 μm. Data are mean ± SEM; **p = 0.0029, ***p = 0.0008 vs. control, ##p = 0.0025. n.s., no significant difference