Fig. 2

Activation of different brain regions by abdominal aversive stimuli in female mice. a, b The insular cortex was divided into three segments aIC, mIC and pIC. Representative histology (a) and quantification (b) of Fos-like immunoreactivity in the left- and right-side along the entire length of A-P axis of the insular cortex after intraperitoneal injection of LiCl (150 mg/kg). c Quantification of Fos-like immunoreactivity in AID, DI, GI, AIV. d, e Representative histology (d) and quantification (e) of Fos-like immunoreactivity in the left and right PVT, ACC, LHA, PBN, CeA, NTS after intraperitoneal injection of LiCl (150 mg/kg). f, g Representative histology (f) and quantification (g) of Fos-like immunoreactivity in the left and right insular cortex after intraperitoneal injection of Cisplatin (4 mg/kg). IC insular cortex, aIC anterior insular cortex, mIC middle insular cortex, pIC posterior insular cortex, PBN parabrachial nucleus, CeA central amygdala, ACC anterior cingulate cortex, NTS nucleus of solitary tract, LHA, lateral hypothalamus, PVT paraventricular thalamic nucleus, AID agranular insular cortex, dorsal part; DI dysgranular insular cortex, GI granular insular cortex, AIV agranular insular cortex, ventral part. n = 8 mice per group. Data were presented as means ± SEM. Turkey’s test analysis between each group as indicated *P < 0.05. Scale bar, 100 μm