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Fig. 3 | Molecular Brain

Fig. 3

From: Activation of acid‐sensing ion channels by carbon dioxide regulates amygdala synaptic protein degradation in memory reconsolidation

Fig. 3

CO2 inhalation does not directly affect proteasome activity. a Schematic of experimental procedure of aversive conditioning (Av. C.), memory retrieval (Ret) and CO2 inhalation, as described previously. Thirty minutes later, brain slices were prepared using a brain matrix, and amygdala isolated by a biopsy punch. Whole tissue lysates were subjected to proteasome activity analysis. b Samples collected from animals receiving aversive conditioning with or without Ret and with or without CO2 are indicated. Proteasome activity in cell lysates was measured by a fluorometric proteasome 20s assay kit. Samples prepared from animals without behavioral treatment served as controls, and a proteasome inhibitor was used to confirm the specificity. c Tissue samples were collected from animals subjected to aversive conditioning and Ret/CO2. Rpt6 phosphorylation was assessed by SDS-PAGE and Western Blot analysis using anti-phospho-Rpt6 and anti-actin antibodies. Values are expressed in mean ± SEM of three independent experiments. ‘n.s.’ indicates not statistically significant by one-way ANOVA with Tukey’s post-hoc multiple comparisons

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