Fig. 1
Phenotypes of gba2 KO medaka. a The Gba2 enzymatic activity in the brains of medaka. The Gba2 activity was almost eliminated in gba2 KO medaka and reduced by 42% in gba2+/− medaka (n = 6 for each genotype). A one-way ANOVA with Tukey's multiple comparison test was performed. b A Kaplan–Meier plot showed no significant differences in the survival among genotypes (n = 24–33 for each genotype). A Log-rank (Mantel-Cox) test was performed. c Analysis of the spontaneous swimming movement. gba2 KO medaka showed a normal locomotor function compared with WT medaka at 6 mpf (n = 6 for each genotype). A two-tailed unpaired Student’s t-test was performed. The bottom images are representative movement tracks for each genotype. d Fertilization rate analysis showed no marked differences among genotypes at 6 pmf (n = 3 for each group). A one-way ANOVA with Tukey's multiple comparison test was performed. n.s. not significant, ***p < 0.001. The bars indicate the mean ± SEM