Fig. 5

Autophagic dysfunction in gba1 KO and gba1/gba2 DKO medaka, but not in gba2 KO medaka. a, b Immunoblot analysis of p62 in the brains at 3 mpf. The expression of p62 was increased in the gba1/gba2 DKO and gba1 KO medaka but not in gba2 KO medaka (n = 6 for each genotype). a, c Immunoblot analysis of LC3-I and LC3-II in the brains at 3 mpf. The LC3-II/I ratio was increased in the gba1/gba2 DKO and gba1 KO medaka compared with the WT and gba2 KO medaka (n = 6 for each genotype). A one-way ANOVA with Tukey's multiple comparison test was performed. n.s. not significant, **p < 0.01, and ***p < 0.001. The bars indicate the mean ± SEM