Fig. 6

Endocochlear potential and immunohistological analysis of spiral ligament (SLi). a Endocochlear potentials for Cdk5rap1-knockout (KO) and littermate control (CNT) mice at 4, 12, 20, or 48 weeks of age. b Schematic representation of the SLi illustrating the five types of fibrocytes. c H&E staining of the SV and SLi of CNT and KO mice at different ages. d Immunohistochemistry of SLi of CNT and KO mice at different ages. Na⁺/K⁺-ATPase α1 (red) for types II, IV, and V. e Immunohistochemistry of SLi of CNT and KO mice at different ages. Connexin 26 (Cx26; red) for type I. f Immunohistochemistry of SLi of CNT and KO mice at different ages. Aquaporin 1 (Aqp1; red) for type III. Scale bar = 100 µm. d’ The modified labeling index (mLI) of Na⁺/K⁺-ATPase α1 observed in CNT and KO mice at different ages. e’ The mLI of Cx26 observed in CNT and KO mice at different ages. f’ The mLI of Aqp1 observed in CNT and KO mice at different ages