Fig. 2

Atg7 deficiency impairs the microglial response to lipopolysaccharide, but not interleukin 4. a Immunoblot analysis of NOS2 protein expression in shAtg7 and shCtrl BV2 cells. The expression of ACTB as housekeeping gene was used as loading control. Cells were treated with LPS (100 ng/ml) for 3 h or 6 h. b Graphs show the quantification for NOS2 versus ACTB protein expression and in shAtg7 and shCtrl BV2 cells. Values are in comparison to shCtrl BV2 after 3 h LPS. c Comparison of Nos2 mRNA expression measured by RT-qPCR in shAtg7 and shCtrl BV2 cells. d Immunoblot analysis of ARG1 protein expression in shAtg7 and shCtrl BV2 cells. The expression of ACTB as housekeeping gene was used as loading control. Cells were treated with IL-4 (10 ng/ml) for 3 h or 6 h. b Graphs show the quantification for ARG1 versus ACTB protein expression and in shAtg7 and shCtrl BV2 cells. Values are in comparison to untreated shCtrl BV2. c Comparison of Arg1 mRNA expression measured by RT-qPCR in shAtg7 and shCtrl BV2 cells. All values are a mean of 3 (b, f) or 4 (c, e) independent experiments ± SEM and considered significant for *p < 0.05, **p < 0.01. n.s., not significant for the indicated comparison