Fig. 6

Frequency distribution of mushroom spines clustered by diameter size in CA1 stratum radiatum of untreated animals (WT and Ts1Cje) or mice treated with rapamycin prenatally (WT RAPA and Ts1Cje RAPA). A Representative images of Golgi staining for WT, Ts1Cje, WT RAPA and Ts1Cje RAPA mice; a typical mushroom spine is also shown. B Distribution of mushroom spines clustered by diameter size. Frequencies are shown as percentages. Center of the first and last bins in the histogram were automatically fixed (using GraphPad Prism software), and bin wide was set to 0.2 μm. The Z-score for two population proportions was calculated between WT vs. Ts1Cje, WT vs. WT RAPA and Ts1Cje vs. Ts1Cje RAPA for each histogram interval. Statistically significant p-values were obtained when comparing WT vs. Ts1Cje, and Ts1Cje vs. Ts1Cje RAPA, (as indicated with asterisks) in the following cases: 0.5 to 0.7 μm interval (bin center 0.6 μm): WT vs. Ts1Cje p-value = 0.011, Ts1Cje vs. Ts1Cje RAPA p-value < 0.001; 0.7 to 0.9 μm interval (bin center 0.8 μm): WT vs. Ts1Cje p-value = 0.045, Ts1Cje vs. Ts1Cje RAPA p-value = 0.002