Fig. 3
Enhanced synaptic plasticity in the hippocampal Schaffer collateral-CA1 pathway of IN-PS cDKO mice. a Enhanced PPF in IN-PS cDKO mice. Averaged PPF is plotted as a function of the inter-stimulus intervals (20-2000 ms). PPF is higher in IN-PS cDKO mice relative to littermate controls (F1, 19 = 8.74; p < 0.01, two-way ANOVA). Insets are representative fEPSP traces evoked by two consecutive stimuli with a 40 ms inter-pulse interval. b Superimposed fEPSP traces of frequency facilitation elicited by 20 Hz stimulus train show greater enhancement in IN-PS cDKO mice relative to controls. c Summary graphs show that synaptic facilitation elicited by stimulus trains is enhanced, and that the enhancement is greater in IN-PS cDKO mice relative to controls (1 Hz: F1, 19 = 21.15, p < 0.001; 5 Hz: F1, 19 = 16.35, p < 0.001; 10 Hz: F1, 19 = 8.75, p < 0.01; 20 Hz: F1, 19 = 7.41, p < 0.05; two-way ANOVA). d Enhanced LTP induced by 5 trains of TBS in IN-PS cDKO mice. Superimposed traces are averages of four consecutive responses 1 min before (1), 7 min and 60 min after (2, 3) TBS induction. Summary graph shows the magnitude of LTP measured during the last 10 min post-induction (51–60 min) in control and IN-PS cDKO hippocampal slices. eNormal AMPA receptor-mediated synaptic transmission in IN-PS cDKO mice. The lines represent the best linear regression fit. The input/output slopes are similar between control (y = 1.27x, R2 = 0.96) and IN-PS cDKO (y = 1.32x, R2 = 0.94) mice (p = 0.46; linear regression). All data represent mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). The number of slices/mice used in each experiment is shown in parentheses