Fig. 10

Verification of specificity of antibodies made against different epitopes of transmembrane proteins. Antibodies made against extracellular (external) epitopes should be localized to the extracellular side of the postsynaptic membrane (a) and the lumen of the ER (c, d), while antibodies made against cytosolic epitopes should be localized to the cytosolic side of the postsynaptic membrane (b) and the cytosolic side of the ER (e, f). a–e are from dissociated hippocampal cultures, and f is from Purkinje soma of perfusion-fixed cerebellum. Scale bars = 100 nm, a & b share the same bar, c-f share the same bar