Fig. 11From: Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brainsPerfusion-fixed mouse brains labeled with Homer 2 (a–c) and Homer 1/2/3 (d). Label for a Homer 2 antibody was specifically localized at the PSDs with very little background elsewhere (a), and correctly situated at the deeper layer of the PSD (b). Additional signals of this antibody were also seen associated with the cytoplasmic side of the nuclear pores (arrows in c). However, another pan Homer antibody was not detected at the nuclear pore (d). Scale bars = 100 nmBack to article page