Fig. 14

Dissociated hippocampal cultures labeled with CaMKII (a, d), chromogranin A (b, e) and two non-specific antibodies (c, f). The clusters of signals in the left two columns showed legitimate multiple signals of the aggregated proteins. The two primary antibodies used in (c, f) turned out to be non-specific, but these images illustrated clumping of the secondary antibody, and therefore, these clusters should not be counted as multiple signals. Scale bars = 100 nm