Fig. 4

Samples in a and b were labeled for SV2 and treated identically except for the silver enhancement step. Samples treated with the Aurion silver enhancement kit for 60 min showed signals of more or less uniform-sized particles (a), while samples treated with the Nanoprobes HQ silver enhancement kit for 6 min showed signals of heterogeneous sizes at a much higher density (b). Sample in c was from a separate experiment where sample was labeled with synaptophysin, another SV membrane protein, and the time of HQ silver enhancement was shortened to 5 min, resulting in smaller sized particles. Scale bar = 100 nm