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Fig. 7 | Molecular Brain

Fig. 7

From: Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains

Fig. 7

Dissociated hippocampal cultures labeled with SV2. Images had more contrast when samples were en bloc stained with UA at pH 5.3 (a) than at pH 6.0 (b). Contrast of the image was even stronger when the sample was treated with UA at pH 5.0 (d), but the silver particles became grey shadows (small arrows in d) when compared to the black particles in samples treated with UA at pH 5.3 (c). Scale bars = 100 nm. a & b shared the same bar, c & d shared the same bar

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