Fig. 8From: Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brainsDissociated hippocampal cultures labeled for Homer 1b/c, a scaffold protein at the PSD [30]. When sections were cut with a diamond knife cleaned with ethanol, the silver particles had a smudged appearance, with numerous fine dots in the vicinity of the particles (a). In contrast, the silver particles appeared intact (b) in sections from the same block that was cut with a diamond knife never cleaned with ethanol. Scale bar = 100 nmBack to article page