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Table 1 Specifics on primary antibodies

From: Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains

Target protein

Location in neurons

Antibody species

& clone number

Sources

Synaptophysin

Synaptic vesicle (SV) membrane

Rabbit polyclonal (pAb)

DAKO (Glostrup, Denmark)

SV2

SV membrane

Mouse monoclonal (mAb), clone 10H3

gift from Dr. Erik S. Schweitzer

VGluT (vesicular glutamate transporter)

SV of glutamatergic terminals

Rabbit pAb

Synaptic Systems (Goettingen, Germany)

synapsin I

SV-associated

Mouse mAb

clone 46.1

Synaptic Systems

α-Synuclein

SV-associated

Mouse mAb

clone 42

BD Biosciences (San Jose, CA, USA)

Bassoon

Presynaptic active zone cytomatrix

Mouse mAb

clone SAP7F407

Stressgen (Victoria, BC, Canada)

GluR2

(AMPA receptor)

Postsynaptic membrane

Mouse mAb

clone 6C4

Millipore (Billerica, MA, USA)

NR2B

(NMDA receptor)

Postsynaptic membrane

Mouse mAb

clone N59/36

NeuroMab (Davis, CA, USA)

NR2A/B

(NMDA receptor)

Postsynaptic membrane

Rabbit pAb

Chemicon (Temecula, CA, USA)

PSD95

Postsynaptic density (PSD)

Rabbit pAb raised against residues 290–307

Custom-made by New England Peptide (Gardener, MA, USA)

synGAP

PSD and cytosolic

Rabbit mAb

clone EPR 2883

Millipore

pan Shank

PSD

Mouse mAb

clone N23B/49

NeuroMab

Shank 2

PSD

Mouse mAb

clone N23B/6,

NeuroMab

Shank 3

PSD

Rabbit pAb

Santa Cruz (Dallas, TX, USA)

Homer 1

PSD

Rabbit pAb

Synaptic Systems

Homer 1b/c

PSD

Rabbit pAb

Synaptic Systems

Homer 2

PSD

Rabbit pAb

Synaptic Systems

Homer 1/2/3

PSD

Rabbit pAb

Synaptic Systems

IRSp53 ab 1

PSD

Mouse mAb

clone L117/1

NeuroMab

IRSp53 ab 2

PSD

Rabbit pAb

Protein Tech Group (Rosemont, IL, USA)

α-CaMKII

PSD and cytosolic

Mouse mAb

clone 6G9(2)

Millipore

Ip3 receptor

Endoplasmic reticulum (ER) membrane

Mouse mAb

clone L24/18

NeuroMab

Ryanodine receptor

ER membrane

Mouse mAb

clone 34C

Affinity BioReagents (Golden, CO, USA)

Chromogranin A

Dense core of dense core granule

Rabbit pAb

gift from Dr. Lee Eiden (NIMH, Bethesda, MD, USA)