Fig. 3
From: Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation
Ribosome associated mitochondrial-related mRNA are altered in NAc MSN subtypes after repeated cocaine. A Illustration of NAc D1-MSN (blue) and D2-MSN (red) subtypes and the RiboTag procedure to isolate ribosome-associated mRNA from MSN subtypes using D1-Cre-RT and D2-Cre-RT mice. HA tagged (green) ribosomes are immunoprecipitated from NAc homogenates using anti-HA coupled to magnetic beads, followed by isolation of ribosome-associated mRNA from D1-MSNs and D2-MSNs. B Top panel: The timeline of cocaine (20 mg/kg, i.p.) or saline injections and NAc tissue collection after 24-h abstinence. Bottom panel: Tfam ribosome-associated mRNA is increased in D1-MSNs Student’s t-test, *p < 0.05; p = 0.07 Nrf2; p = 0.052 Tomm20; D1-MSN saline group (n = 16 females, 8 male; pooled samples 4 and 2, respectively); D1-MSN cocaine group (n = 8 female, 16 males; pooled samples 2 and 4, respectively). C Polγ, Tfam, Tfb1 and Tomm20 expression is reduced in D2-MSNs after repeated cocaine (7 days, 20 mg/kg). Student’s t-test, *p < 0.05; **p < 0.01;***p < 0.001; D2-MSN saline group (n = 8 female, 16 male; pooled samples 2 and 4, respectively) and D2-MSN cocaine group (n = 12 female, n = 12 male; pooled samples 3 and 3, respectively); Error bars, SEM