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Fig. 2 | Molecular Brain

Fig. 2

From: Cannabinoid receptors distribution in mouse cortical plasma membrane compartments

Fig. 2

CB receptors PM compartmental distribution in mouse cortical tissue. A Aliquots of fractions collected from top to bottom of the gradient were subjected to SDS-PAGE isolation and analyzed by western blotting with antibodies directed against CB1 receptor, CB2 receptor, flotillin-1, caveolin-1, PrPc and transferrin receptor. To investigate purity of the fractionation process, the PVDF membranes were later stripped and probed with Cytochrome C (mitochondrial marker) and SCD1 (endoplasmic reticulum marker). Mouse cortical lysate (40 µg) was loaded on each gel as positive control. B Quantification of percent of CB1 or CB2 receptor present in each fraction relative to total receptor across all fractions within sample was performed using ImageJ software. All these experiments were repeated 5 times. Data are presented as mean ± S.E.M

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